Facility Instrumentation
The Facility is equipped with three research-grade cell sorters capable of sorting 100 - 15000 events/second based on multiparameter analysis of cells characteristics (size, density, and fluorescence intensities of multiple colors). Additionally, two analyzers are available for self-service analysis after users have completed training provided by the facility.
A completed Flow Cytometry Project Form must be submitted prior to any instrument usage.
- Compatible with BSL2+ conditions
- Capable of 14 color analysis and sorting
- Excitation wavelengths of 405 nm, 488 nm, 561, and 633 nm (PDF Configuration)
- Sample deposition for sorting into a variety of plates and tubes including:
- Culture plates (6, 12, 24, 48, or 96-well)
- PCR plates
- 4 way tube sorting into 5mL or 1.5mL tubes
- 2-way sorting into 15mL tubes
- Available nozzle sizes: 70 µm, 85 µm, 100 µm
- Cuvette system allows for better fluorescence sensitivity
- Sorting droplet is dispensed at an angle for less precision into smaller welled plates
- Onboard software: DiVa
- Compatible with BSL2 conditions
- Capable of 15 color analysis and sorting
- Excitation wavelengths at 405 nm, 488 nm, 561 nm, and 638 nm (PDF Configuration)
- Sample deposition for sorting into a variety of plates and tubes including:
- Culture plates (6, 12, 24, 48, or 96-well)
- PCR plates
- 4 way tube sorting into 5 mL or 1.5 mL tubes
- 2-way sorting into 15 mL tubes
- Available nozzle sizes: 100 µm
- Cuvette system for better fluorescence sensitivity
- Sorting droplet is dispensed at an angle for less precision into smaller welled plates
- Onboard software: CytExpert
- Compatible with BSL2 conditions
- Capable of 9 color analysis and sorting
- Excitation wavelengths at 355 nm (UV), 407 nm, 488 nm, 561 nm, 633 nm (PDF Configuration)
- Sample deposition for sorting into a variety of plates and tubes including:
- Culture plates (6, 12, 24, 48, or 96-well)
- PCR plates
- 384-well plates
- 60-well Terasaki plates
- 4 way tube sorting into 5 mL or 1.5 mL tubes
- 2-way sorting into 15 mL tubes
- Available nozzle sizes: 70 µm, 100 µm
- Jet-in-air system for better post-sort viability
- Sorting droplet is dispensed straight down for more precision into smaller welled plates
- Onboard software: WinList from Verity Software House
- Large particle sorting of ~30 μm to 800 μm
- Colinear lasers at 488 nm and 561 nm
- Filters: 512/25, 543/22, 615/25
- FOCA 500 and 1000 available
- Sample deposition for sorting into a variety of plates and tubes including:
- Culture plates (6, 12, 24, 48, or 96-well)
- PCR plates
- 384-well plates
- 1.5 mL, 5 mL, 15 mL and 50 mL tubes
- Sorting droplet is dispensed straight down for more precision into smaller welled plates
- Compatible with BSL2 conditions
- Capable of 13 color analysis
- Excitation wavelengths at 405 nm, 488 nm, 561 nm, and 638 nm (PDF Configuration)
- Equipped with single-tube or 96-well sampler
- Onboard software: CytExpert
- Compatible with BSL2 conditions
- Capable of 8 color analysis
- Excitation wavelengths at 405 nm, 488 nm, and 561 nm (PDF Configuration)
- Equipped with single-tube or 96-well sampler and chill blocks
- Onboard software: MACSQuantify
- Owned by the Elowitz lab but resides in the facility for shared use
- Pipettes
- Sonicators
- Vortex Mixers
- Oven
- Microcentrifuge
- Beckman Coulter Allegra 6R Centrifuge
- Equipped with buckets for plates, 15mL tubes, and 50mL tubes