Common Recommendations
Shelley's Buffer – HBSS:
- HBSS without phenol red, Ca2+ or Mg2+
- 10mM HEPES buffer (be careful to use the cell culture variety and not just a chemical buffer)
- DNAse1 (50ug/ml) from Roche (added the same day as the sort, to minimize aggregation due to cell death)
- 1mM MgCl2
- BSA Fraction V (2.5mg/ml)
- Be sure to check your pH (BSA is acidic)
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