skip to main content
Home  /  Getting Started  /  Common Recommendations

Common Recommendations

Shelley's Buffer – HBSS:

  • HBSS without phenol red, Ca2+ or Mg2+
  • 10mM HEPES buffer (be careful to use the cell culture variety and not just a chemical buffer)
  • DNAse1 (50ug/ml) from Roche (added the same day as the sort, to minimize aggregation due to cell death)
  • 1mM MgCl2
  • BSA Fraction V (2.5mg/ml)
    • Be sure to check your pH (BSA is acidic)

Useful Links:

Trypsin inhibitor

Falcon tube cap strainers

Celltrics strainers (50um)

5ml Tubes